EFFECT OF VEGETABLE COMPOUNDS ON HEMOLYTIC ACTIVITY OF MICROORGANISMS

Zolotarev Pavel Nikolaevich, Сandidate of medical science, associate professor, sub-department of pathology and morphology, Medical University «REAVIS» (227 Chapaevskaya street, Samara, Russia), zolotareff@list.ru

576.8.06; 616-018-002.3: 615.322

Background. The specific nature of biological feature changes in microorganisms requires modern drugs to have bacteriostatic and bactericidal effects, as well as an opportunity to regulate the biological potential of a causative agent. The present work is devoted to studying a modification of the hemolytic activity of microorganisms that cause pyoinflammatory diseases under the influence of phytosubstances.
Materials and methods. The authors used 31 bacteria strains, extracted from wounds at pyoinflammatory diseases of soft tissues, and museum strains as the research material. The microorganisms were segregated and identified using an automatic bacteriological analyzer. In order to study the influence of phytosubstances on hemolytic activity the bacteria were cultivated in nutrient broths with subinhibitory concentrations of substances. The obtained data were processed by the methods of descriptive statistics and presented in the form of a confidence interval.
Results. It has been established that the maximum inhibitory effect on the hemolytic activity of Staphylococcus spp. was exerted by lilac extract, poplar gemmae extract, St. John’s-wort extract, tarragon sagebrush extract, carnation essential oil, carnation carbon dioxide extract and the second fraction thereof. The maximum reduction of the hemolytic activity of Streptococcus spp. has been revealed under the influence of poplar gemmae extract, tarragon sagebrush extract, sylibum liquid extract, carnation carbon dioxide extract and the second and third fractions thereof. The Pseudomonas aeruginosa strains decreased the hemolytic activity to the max under the influence of Melissa extract, poplar gemmae extract, birch extract, tarragon sagebrush extract, as well as the first and second fractions of carnation carbon dioxide extract.
Conclusions. It has been established that phytosamples exert both stimulating and inhibitory impacts on hemolytic activity. The multidirectional effect depends both on bacteria species belonging, as well as on the nature of phytosubstances. Experimental studying of regulation of hemolytic activity of microorganisms by phytosubstances proves application expediency of the present samples in clinical practice as auxiliary means both at the stage of etiotropic treatment and for prevention of a number of infectious diseases.

factors of pathogenicity, hemolytic activity, phytosubstances, inflammatory diseases.

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